Synthesis of Block Oligonucleotides
- 12 March 1965
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 147 (3663) , 1310-1311
- https://doi.org/10.1126/science.147.3663.1310
Abstract
The concentration of sodium chloride strongly influences primed polymerization of nucleoside diphosphates by polynucleotide phosphorylase. High concentrations of NaCl allow the addition of only a few nucleotide residues onto the 3' end of the primer. When pure oligonucleotides are used as primers and when the product is fractionated, pure block oligonucleotides can be obtained.Keywords
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