The Effect of Organic Solvents on the Appearance of Bacterial Nuclei
- 1 January 1953
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 28 (6) , 313-317
- https://doi.org/10.3109/10520295309105564
Abstract
Treatment of bacterial smears with organic solvents was found to produce a typical appearing nuclei which could lead to false interpretation of nuclear events. The crystal violet nuclear stain, which does not utilize organic solvents or acid hydrolysis, was used as a basis of comparison. Acid hydrolysis also was observed to affect the appearance of the nucleus. It is concluded that caution should be used in interpreting nuclear structure and activity in bacterial cells, especially when organic solvents or acid hydrolysis are involved in the staining technic.Keywords
This publication has 4 references indexed in Scilit:
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- Crystal Violet as A Nuclear Stain for Gaffkya Tetragena and Other BacteriaStain Technology, 1952
- A NEW CYTOLOGICAL BASIS FOR BACTERIAL GENETICSCold Spring Harbor Symposia on Quantitative Biology, 1951
- A Staining and Dehydrating Procedure for the handling of MicroörganismsStain Technology, 1951