Amikacin resistance mediated by multiresistance transposon Tn2424
- 1 August 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 155 (2) , 755-760
- https://doi.org/10.1128/jb.155.2.755-760.1983
Abstract
Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, which conferred higher levels of resistance in Pseudomonas aeruginosa than in Escherichia coli. A restriction analysis and cloning experiments resulted in a physical and functional map of Tn2424. Comparison by a heteroduplex technique revealed that Tn2424 includes the total sequence of Tn21 and two additional DNA fragments that are 1.8 and 4 kilobases long.This publication has 28 references indexed in Scilit:
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