Karyotypic analysis of two L1210 murine leukemia lines growing in vivo and in vitro.

Abstract
The comparative results of karyotypic investigations of murine leukemia cells L1210, growing in vivo and in vitro are presented. The frequency of normal chromosomes in both lines differs as follows: in the in vivo line monosomia of chromosomes of pairs 4, 17 and 19 is frequent, while the in vitro derivate shows monosomia or even complete absence of chromosome X. Both lines have 2 chromosomal markers characteristic for leukemia L1210. The parent line had 2 metacentric markers; 1 from 5-12 fusion, being common to both lines, whereas the other from 9-marker M4 which is exclusive to in vivo cells.