An Unusual Isotope Effect on Substrate Inhibition in the Oxidation of Arachidonic Acid by Lipoxygenase
- 1 July 2003
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (30) , 8988-8989
- https://doi.org/10.1021/ja035977t
Abstract
Soybean lipoxygenase catalyzes the oxidation of arachidonic acid to 15S-HPETE. The reaction displays strong substrate inhibition with unlabeled substrate but no discernible substrate inhibition with arachidonic acid labeled with deuterium at C13, the site of hydrogen/deuterium atom abstraction. The unusual behavior is due primarily to a large kinetic isotope effect on Km,O2 as a result of the strong selection against deuterium in the abstraction step.Keywords
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