LOCALIZATION OF ALPHA-FETOPROTEIN IN LIVER-TISSUE OF RATS DURING POSTNATAL-DEVELOPMENT - COMPARISON OF IMMUNOFLUORESCENT AND AUTORADIOGRAPHIC METHODS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 23  (1) , 1-10
Abstract
Localization of .alpha.-fetoprotein was followed in the liver of rats of the Wistar strain from birth up to 37th day of life, and its presence was detected by immunofluorescence and autoradiography. The former showed .alpha.-fetoprotein to be localized in hepatocytes and the number of positive hepatocytes declined proportionally with that of .alpha.-fetoprotein concentration in the serum. In newborn rats almost all hepatocytes were positive. During the course of subsequent development of the liver tissue the number of positive hepatocytes decreased and time of the formation of the lobular structure only certain groups of lobes remain positive. The number of positive cells gradually diminishes from the preiphery toward the vena centrails. The autoradiographic method revealed only small groups of individual cells to be .alpha.-fetoprotein, positive, without any specific localization. [.alpha.-Fetoprotein reappears in hepatocellular carcinoma and ovarian teratoblastoma.].

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