Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena
- 1 July 1993
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 9 (1) , 77-84
- https://doi.org/10.1111/j.1365-2958.1993.tb01670.x
Abstract
Summary: The spatially patterned differentiation of hetero‐cysts in the filamentous cyanobacterium Anabaena requires a functional hetR gene. Transcriptional fusions to luxAB show that hetR is transcribed at a low level throughout the filament when Anabaena is grown with combined nitrogen, and that induction of the gene begins within 2 h following nitrogen deprivation. By 3.5 h, induction is localized to spaced foci. By 6h, there is an overall induction of at least threefold in whole cultures, reflecting at least a 20‐fold increase within spatially separated cells. The induction requires the presence of a functional hetR gene, indicating that hetR is autoregulatory. Full induction of a heterocyst structural gene, hepA, also requires a functional hetR locus.Keywords
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