Purification of Giardia Muris Trophozoites by Using Nylon Fiber Columns
- 1 January 1980
- journal article
- research article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 29 (1) , 12-15
- https://doi.org/10.4269/ajtmh.1980.29.12
Abstract
A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide®, specific gravity 1.10, followed by incubation on a nylon fiber column at 37°C for 120 min, yielded up to 15 × 106 viable purified trophozoites per infected mouse.This publication has 3 references indexed in Scilit:
- ANIMAL-MODEL OF HUMAN DISEASE - GIARDIASIS1978
- Giardiasis in the Mouse: An Animal ModelGastroenterology, 1976
- Giardia lamblia: Isolation and axenic cultivationExperimental Parasitology, 1976