Molecular analysis of the transcripts encoding the light-harvesting chlorophyll a/b protein in Euglena gracilis: unusual size of the mRNA
- 1 December 1987
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 12 (8) , 611-616
- https://doi.org/10.1007/bf00368064
Abstract
A cDNA library made from Euglena polyadenylated RNA has been constructed in the expression vector λgt11. The library was screened with antibodies directed against the LHCP apoproteins. Several putative LHCP clones were isolated and analyzed. We present the nucleotide and derived amino-acid sequence of an 800 by long insert containing a poly A tail. On an aminoacid basis, the Euglena LHCP shows a 60% homology with the corresponding pea gene. Northern blot analysis revealed an unusual size of 7.5 kb. This extremely large mRNA is also recognized by a LHCP probe from Chlamydomonas. Hybrid-selected translation produces 2 proteins of 90–110 kd equivalent in size of the polypeptides immunoprecipitated from the translation products of polyadenylated RNAs.This publication has 35 references indexed in Scilit:
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