EFFECT OF HEAT AND SELECTED CHEMICAL DISINFECTANTS UPON INFECTIVITY OF SPORES OF BACILLUS-PILIFORMIS (TYZZERS DISEASE)

  • 1 January 1980
    • journal article
    • research article
    • Vol. 30  (2) , 192-196
Abstract
Spores of B. piliformis of rabbit origin were harvested from the yolk sac of previously inoculated hen''s eggs and subjected to various heat or chemical disinfectant treatments. Subsequently, spores were tested for infectivity in embryonated eggs inoculated via the yolk sac route and others were treated with trypsin and then tested in embryonated eggs. The spores were not affected by the heat treatment of 60.degree. C but were rendered non-infective with treatments of 70 and 80.degree. C. Infectivity was not restored by treatment with trypsin. Infectivity was not lost when spores were treated with a phenolic germicidal detergent, ethanol or either of 2 quaternary ammonium compounds containing 9 or 17% benzalkonium chloride. A graded effect was observed with formaldehyde solution and an iodophor. Spores were rendered noninfective after treatment with peracetic acid (1.0%) and a wetting agent, sodium alkylarylsulfonate or sodium hypochlorite solution (0.3%) for 5 min and infectivity was not restored by trypsin treatment. The probable means of transmission of B. piliformis was discussed and sodium hypochlorite solution (0.3%) was recommended as a surface disinfectant in animal facilities as an aid to the prevention and control of Tyzzer''s disease.

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