Cysteine-protease involved in male chromatin remodeling after fertilization co-localizes with α-tubulin at mitosis
- 5 August 2004
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 202 (2) , 602-607
- https://doi.org/10.1002/jcp.20157
Abstract
We postulated an essential role for a cysteine-protease in sea urchins sperm histones degradation which follows fertilization. We now report the purification of this enzyme, the determination of its N-terminal amino acid sequence and the localization of the protein with antibodies generated against this amino-terminal peptide. The immunofluorescence data confirmed the presence of this enzyme in the nucleus of unfertilized eggs. After fertilization labeling is observed both in female and male pronuclei suggesting a rapid recruitment of the enzyme to the male pronuclei. Interestingly, we have found that this cysteine-protease persists in the nucleus of the zygotes during S phase of the cell cycle and co-localizes with alpha-tubulin that organizes the mitotic spindle during the initial embryonic cell division.Keywords
Funding Information
- FONDECYT (011073)
- DIUC (200.031.088-10)
- International visiting exchange: ECOS France/CONICYT Chile and Royal Society U.K./CONICYT Chile
This publication has 17 references indexed in Scilit:
- Chromatin remodeling during sea urchin early development: molecular determinants for pronuclei formation and transcriptional activationGene, 2003
- Cytoplasm of sea urchin unfertilized eggs contains a nucleosome remodeling activityJournal of Cellular Biochemistry, 2001
- Membrane Fusion Events during Nuclear Envelope AssemblyPublished by Springer Nature ,1999
- Poly(ADP-ribosylation) protects maternally derived histones from proteolysis after fertilizationBiochemical Journal, 1999
- Cdk2 Activity Is Dispensable for the Onset of DNA Replication during the First Mitotic Cycles of the Sea Urchin Early EmbryoDevelopmental Biology, 1998
- Identification of a cysteine protease responsible for degradation of sperm histones during male pronucleus remodeling in sea urchinsJournal of Cellular Biochemistry, 1997
- Cloning, expression, purification, and characterization of the major core protein (p26) from equine infectious anemia virusBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1997
- Immunobiochemical evidence for the loss of sperm specific histones during male pronucleus formation in monospermic zygotes of sea urchinsJournal of Cellular Biochemistry, 1991
- Poly(ADP‐ribosylation) of atypical CS histone variants is required for the progression of S phase in early embryos of sea urchinsJournal of Cellular Biochemistry, 1991
- Two Drosophila beta tubulin isoforms are not functionally equivalent.The Journal of cell biology, 1990