Activation of phospholipase A in rabbit erythrocyte membranes by a novel hemolytic toxin (H-toxin) of
- 15 March 1990
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 68 (3) , 319-322
- https://doi.org/10.1016/s0378-1097(05)80061-8
Abstract
A primary effect of a novel H-toxin of Clostridium septicum on the hemolysis of rabbit erythrocytes was shown to be the activation of phospholipase A2 (PLA2) associated with rabbit erythrocyte membranes by 20-fold that of controls. Furthermore, the activation of PLA2 induced by the H-toxin was enhanced in the presence of NAD. The H-toxin itself had no PLA2 activity. On the contrary, the H-toxin bound to palmitic acid at a molar proportion of 1:1 and lost its hemolytic activity. The PLA2 was not activated by the H-toxin bound to palmitic acid. These results suggest that activation of the PLA2 is responsible for development of the hemolytic activity of the H-toxin.Keywords
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