Par-1 regulates stability of the posterior determinant Oskar by phosphorylation
- 15 April 2002
- journal article
- Published by Springer Nature in Nature Cell Biology
- Vol. 4 (5) , 337-342
- https://doi.org/10.1038/ncb782
Abstract
Par-1 kinase is critical for polarization of the Drosophila melanogaster oocyte and the one-cell Caenorhabditis elegans embryo. Although Par-1 localizes specifically to the posterior pole in both cells, neither its targets nor its function at the posterior pole have been elucidated. Here we show that Drosophila Par-1 phosphorylates the posterior determinant Oskar (Osk) and demonstrate genetically that Par-1 is required for accumulation of Osk protein. We show in cell-free extracts that Osk protein is intrinsically unstable and that it is stabilized after phosphorylation by Par-1. Our data indicate that posteriorly localized Par-1 regulates posterior patterning by stabilizing Osk.Keywords
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