Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a new Labeling Procedure for a Cationic Spin Label, CAT-16
- 1 July 1988
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 21 (7) , 1131-1140
- https://doi.org/10.1080/00032718808055501
Abstract
A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-l-n-hexadecyl)ammonium-2,2,6,6-tetramethylpiperidine-l-oxyl iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer arc primary.Keywords
This publication has 11 references indexed in Scilit:
- Electron spin resonance (ESR) studies of skeletal protein interactions in human erythrocyte membranes exposed to polyanions and in membranes prepared from inositol hexaphosphate (IHP)-incorporated low-affinity erythrocytesBiochemical and Biophysical Research Communications, 1987
- Conformational alterations within the glycocalyx of erythrocyte membranes studied by spin labellingBiochimica et Biophysica Acta (BBA) - Biomembranes, 1986
- ESR studies of the erythrocyte membrane skeletal protein network: Influence of the state of aggregation of spectrin on the physical state of membrane proteins, bilayer lipids, and cell surface carbohydratesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1985
- BIOENERGETIC STUDIES OF CELLS WITH SPIN PROBESAnnals of the New York Academy of Sciences, 1983
- INDUCED ALTERATIONS IN THE PHYSICAL STATE OF SIALIC ACID AND MEMBRANE PROTEINS IN HUMAN ERYTHROCYTE GHOSTS: IMPLICATIONS FOR THE TOPOLOGY OF THE MAJOR SIALOGLYCOPROTEINAnnals of the New York Academy of Sciences, 1983
- Effects of phytohaemagglutinin, wheat-germ agglutinin, and concanavalin-A on the physical state of sialic acid and membrane proteins in human erythrocyte ghosts: A spin label studyLife Sciences, 1982
- Spin Labeling in DiseasePublished by Springer Nature ,1982
- The band 3 protein of the human red cell membrane: A reviewJournal of Supramolecular Structure, 1978
- On the use of the spin labeling technique in the study of erythrocyte membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- SOLUBILIZATION AND PARTIAL CHARACTERIZATION OF A PHYTOHEMAGGLUTININ RECEPTOR SITE FROM HUMAN ERYTHROCYTESProceedings of the National Academy of Sciences, 1969