RNA-DNA Hybridization: Demonstration in a Mammalian System of Competition by Preincubation
- 9 August 1968
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 161 (3841) , 570-571
- https://doi.org/10.1126/science.161.3841.570
Abstract
Nonspecific intermolecular interactions obscure the interpretation of competition experiments involving hybridization of DNA and RNA in mammalian systems when the labeled RNA/DNA ratio is low. The effect of these interactions can be minimized by choosing a sufficiently high ratio of labeled RNA/DNA. No serious effect of nonspecific interactions is observed in bacteriophage systems, even at very low labeled RNA/DNA ratios.Keywords
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