Production of multivalent extracellular filtrates of Staphylococcus aureus

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Abstract
None of the procedures currently used for producing cell-free filtrates provided preparations containing all of the extracellular products that the culture was able to produce. Even the membrane-filter technique failed to yield such preparations unless the cultures were grown on dialysis membranes laid over Brain Liver Heart Agar plates at 37 C for 48 h in an atmosphere containing 10% CO2 in air and the growth from 25 such membranes was harvested in 12.5 ml of phosphate-buffered saline. These preparations contained detectable quantities of coagulase, phosphatase, DNAse, RNAse, lipase, esterase, egg yolk factor, lysozyme, gelatinase, staphylokinase, and caseinase and were able to hemolyze human, horse, rabbit, and sheep erythrocytes. Broth and semisolid cultures, however, yielded more units of beta hemolysin than did the membrane cultures.

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