Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (2) , 797-811
- https://doi.org/10.1093/nar/15.2.797
Abstract
The dissociation kinetics of 19 base paired oligonucleotide-DNA duplexcontaining a various single mismatched base pair are studied on dried agarose gels. The kinetics of the dissociation are first order under our experimental conditions. The incorporation of a single mismatched base pair destabilizes the DNA duplexes to some extent, the amount depending on the nature of the mismatched base pair. G-T and G-A mismatches slightly destabilize a duplex, while A-A, T-T, C-T and C-A mismatches significantly destabilize it. The activation energy for the overall dissociation processes for these oligonucleotide-DNA duplexes containing 19 base pairs is 52 ± 2 Kcalmol−1 as determined from the slope of Arrhenius plot.Keywords
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