Adaptation of Agaricus campestris (NRRL 2334) to a peat extract medium

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Abstract
The utilization of peat extracts as a substrate source in the submerged growth of fungal mycelium has been investigated. Successive shake flask fermentations were carried out to adapt Agaricus campestris NRRL 2334 to growth on acid extracts of milled sphagnum peat as the only nutrient source. The cultivations were conducted in 250-mL flasks with 100 mL of medium incubated for 6 days at 24 °C and 150 rpm at three different values of initial pH, 5.0, 6.0, and 7.0, and at two different values of inoculum ratio, 5 and 10% (v/v). A progressive increase in the concentration of peat extract in the culture medium, expressed by increasing values of the total carbohydrate concentration of the peat extract in successive steps, resulted in a better adaptation than that obtained after successive transfers in nondiluted peat extract. An initial step of cultivation in a glucose–yeast extract medium was required for better adaptation. The results, reported as dry biomass produced to total carbohydrate consumed and to initial total carbohydrate, suggest that growth-inhibitory substances are present in the peat extract and also are produced during the fermentation.

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