Prognostic value of Cathepsin D expression in breast cancer: Immunohistochemical assessment and correlation with radiometric assay
- 1 April 1994
- journal article
- research article
- Published by Elsevier in Annals of Oncology
- Vol. 5 (4) , 329-336
- https://doi.org/10.1093/oxfordjournals.annonc.a058836
Abstract
The prognostic significance of Cathepsin D and optimal methodologies to measure Cathepsin D in breast cancers are controversial. Quantitative (immunoradiometric) and semiquantitative (immunohistochemical) assays for Cathepsin D expression were compared using 25 breast carcinomas. Immunohistochemical Cathepsin D results were derived using 3 different anti-Cathepsin D antibodies and significant associations between immunohistochemical and radiometric Cathepsin D data were observed for each reagent. Immunohistochemical analysis of Cathepsin D expression was performed on nearly 500 fixed-embedded archival breast cancers with long-term patient follow-up using 2 anti-Cathepsin D antibodies (CDR2-11/23, IC11). The immunohistochemical reagents recognized generally overlapping subsets of Cathepsin D positive tumors (correlation co-efficient 0.54; p − 0.00016). Correlations between Cathepsin D data and clinical, histologic or biologic features differed for each antibody. For the node-negative patient subset, Cathepsin D immunopositivity correlated with erbB-2 and stress-response protein 27 overexpression but not survival. Cathepsin D positivity was associated with subsequent distant metastasis and estrogen receptor positivity in node positive patients. Univariate analysis of all patients suggested that Cathepsin D immunopositivity may be predictive of a reduced metastasis-free but not overall survival. Multivariate analysis, however, failed to confirm an independent prognostic value for Cathepsin D in breast cancer patients. These data do not confirm an independent prognostic significance for Cathepsin D using immunohistochemical methods on breast cancers.Keywords
This publication has 29 references indexed in Scilit:
- Prognostic value of PS2 and cathepsin D in 710 human primary breast tumors: multivariate analysis.Journal of Clinical Oncology, 1993
- Prognosis and treatment decisions in patients with breast cancer without axillary node involvementCancer, 1992
- Prognostic value of total cathepsin D in breast tumorsBreast Cancer Research and Treatment, 1991
- Prognostic relevance of cathepsin D versus oestrogen receptors in node negative breast cancersEuropean Journal of Cancer and Clinical Oncology, 1991
- Cathepsin D and Prognosis in Breast CancerNew England Journal of Medicine, 1990
- Prognostic significance of the estrogen-regulated protein, cathepsin D, in breast cancer. An immunohistochemical studyCancer, 1990
- CATHEPSIN D: AN INDEPENDENT PROGNOSTIC FACTOR FOR METASTASIS OF BREAST CANCERThe Lancet, 1989
- The 52-kDa estrogen-induced protein secreted by MCF7 cells is a lysosomal acidic proteaseBiochemical and Biophysical Research Communications, 1986
- Autocrine Growth Stimulation of the MCF 7 Breast Cancer Cells by the Estrogen-Regulated 52 K Protein*Endocrinology, 1986
- A secreted glycoprotein induced by estrogen in human breast cancer cell linesCell, 1980