DNA structures recognized by the human UV-DDB protein
Open Access
- 1 November 1999
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Symposium Series
- Vol. 42 (1) , 265-266
- https://doi.org/10.1093/nass/42.1.265
Abstract
DNA recognition by the human UV-damaged DNA-binding (UV-DDB) protein was characterized. By circular permutation analyses, DNA duplexes containing the (6-4) photoproduct and the abasic site analog were found to be bent at angles of 54° and 57°, respectively, when they formed a complex with this protein. Although kinked NMR structures have been reported, fluorescence resonance energy transfer experiments revealed that these duplexes had no intrinsic bend. These results suggest that the UV-DDB protein binds DNA that can be bent easily at the above angle.Keywords
This publication has 0 references indexed in Scilit: