ß‐Subunit of Escherichia coli F1‐ATPase
- 29 June 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 218 (2) , 222-226
- https://doi.org/10.1016/0014-5793(87)81050-5
Abstract
A mutant strain KF87 of E. coli with a defective ß‐subunit (Ala‐151 → Val) of F1‐ATPase was isolated. The mutation is within the conserved sequence (G‐X‐X‐X‐X‐G‐K‐T/S) of nucleotide‐binding proteins. The mutant F1‐ATPase had a much higher rate of uni‐site hydrolysis of ATP than the wild type, and about 6% of the wild‐type multi‐site activity. The mutant enzyme showed defective transmission of conformational change(s) between the ligand‐ and aurovertin‐binding sites.Keywords
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