Synthesis of Placental Proteins by the Human Placenta Perfused in vitro

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Abstract
Fresh human placental tissue from deliveries at term was perfused in closed circulation on both the maternal and fetal side. At given times during the 4-hour perfusion period, the amount of human chorionic gonadotropin, human placental lactogen, pregnancy-specific Β1-glycoprotein and pregnancy-associated plasma protein A was determined in the perfusing buffer. These placental proteins were also quantified in the placental tissue before and after perfusion. Calculations showed that all placentae (n = 3) were able to synthesize all 4 proteins tested.

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