Further Studies of Specificity of Antibodies Contained in Antiserum against Poliovirus

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Abstract
Antigenic components of Mahoney strain (poliovirus type 1) involved in virus neutralization reaction were analyzed with mutant Mahoney strains resistant to inhibitors in equine serum (inhibitor-resistant mutants) by means of the kinetic neutralization test. Absorption of [rabbit] anti-Mahoney serum with 5 inhibitor-resistant mutants yielded sera with different antibodies, of which 3 had distinct specificities and 2 specificities possibly partly related to 1 of those 3 sera. Stepwise selection of Mahoney variants resistant to 1, 2, 3 and 4 different inhibitors resulted in gradual deviation of its antigenic composition from that of the original strain. The possible presence of 3 distinct antigenic determinant sites on the surface of Mahoney strain was indicated.