BACTERIOLYSIS OF THE PSEUDOMONADS: I. AGENTS POTENTIATING LYSIS

Publisher Website
Google Scholar
Abstract
The lytic spectrum of 10 species of pseudomonads in systems employing disodium EDTA, sodium deoxycholate, isoamyl alcohol, ethanol, thymol, or lysozyme was determined. Sodium deoxycholate and EDTA were the most active agents. Isoamyl alcohol, ethanol, thymol, and lysozyme elicited only slight lytic responses. EDTA, isoamyl alcohol, ethanol, and thymol when tested with lysozyme gave increased lysis. Synergistic or additive responses were observed. When phosphate buffer was used as the diluent, lysis was increased in all systems. The order of lytic sensitivity of the Pseudomonas species was putrefaciens, very sensitive; aeruginosa, aureofaciens, chlororaphis, and fluorescents, sensitive; caviae, diminuta, and fragi, moderately resistant; andropogonis and pseudomalei, most resistant. Lytic responses were shown to be consistent among strains of the same species and were found to be stable during a 2 year test period.

This publication has 12 references indexed in Scilit: