Sulfhydrylcellulose: a new medium for chromatography of mercurated polynucleotides
- 21 July 1981
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 20 (15) , 4243-4246
- https://doi.org/10.1021/bi00518a001
Abstract
A new medium, sulfhydrylcellulose, for affinity chromatography of mercurated polynucleotides, was synthesized. It is the product of reaction between aminoethylcellulose and N-acetylhomocysteine thiolactone. Sulfhydrylcellulose carries up to 90 .mu.mol of SH groups/g and is inexpensive, easy to prepare and stable. Because it binds mercurated RNA specifically and reversibly and exhibits no size discrimination, sulfhydrylcellulose should have wide applications.This publication has 2 references indexed in Scilit:
- Transcription of adenoviral genetic information in isolated nuclei. Characterization of viral RNA sequences synthesized in vitro.Journal of Biological Chemistry, 1980
- Analysis of in vitro transcription of duck reticulocyte chromatin using mercury-substituted ribonucleoside triphosphatesBiochemistry, 1977