Declining Expression of Neprilysin in Alzheimer Disease Vasculature: Possible Involvement in Cerebral Amyloid Angiopathy

Abstract
Molecular, genetic, and pharmacological studies have shown that neprilysin (also called NEP) catabolizes amyloid β peptides (Aβ) in healthy conditions. However, in Alzheimer disease (AD), Aβ accumulates forming senile plaques in brain parenchyma and amyloid deposition around blood vessels. In this study, we tested at cellular level the relationship between neprilysin and Aβ in human healthy and AD brain. Our results provided evidence for declining levels of neprilysin in AD brains as compared to healthy controls in parallel with increasing deposition of Aβ. In hippocampus of AD individuals we observed a significant down-regulation of neprilysin expression in pyramidal neurons, consistent with the possibility that neprilysin controls the level of Aβ accumulation and plaque formation in this area. In the cortex and leptomeninges, neprilysin was expressed in the smooth muscle cells of blood vessels. In sections from AD patients we observed a clear inverse relationship between neprilysin and Aβ peptide levels in the vasculature, implicating neprilysin in cerebral amyloid angiopathy.