A nonviral minicircle vector for deriving human iPS cells
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- 7 February 2010
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 7 (3) , 197-199
- https://doi.org/10.1038/nmeth.1426
Abstract
Simple minicircle vectors carrying four reprogramming factors induce pluripotency in adult human adipose stem cells and in neonatal fibroblasts without integration into the genome. Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.Keywords
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