Effect of human recombinant interleukin-Iα on release of prostacyclin from human endothelial cells

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Abstract
Incubation of human recombinant IL-1.alpha. (hrIL-1.alpha.) with cultured human endothelial cells induced a dose- and time-dependent increase in the release of prostacyclin (PGI2). Above a dose of hrIL-1.alpha. 0.05 units/ml and following a variable lag phase of between 2 and 4 h, PGI2, release (measured as the stable hydrolysis product 6-keto-prostaglandin F1.alpha.) was detected in the culture supernatant and levels continued to rise throughout a 48-h incubation. The release of PGI2 required the continued presence of hrIL-1.alpha., did not demonstrate tachyphylaxis and was not reduced by pre-incubation with the protein synthesis inhibitors cycloheximide, tunicamycin and actinomycin or by the calmodulin antagonist trifluoroperazine. The relationship of these results to ultraviolet radiation induced erythema is discussed.

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