Immunosorbent for Selective Removal of Lipoprotein (a) from Human Plasma: In Vitro Study
- 1 April 1991
- journal article
- Published by Wiley in Artificial Organs
- Vol. 15 (2) , 136-140
- https://doi.org/10.1111/j.1525-1594.1991.tb00771.x
Abstract
Sheep polyclonal anti-lipoprotein (a) [anti-Lp(a)] antibodies were used for immunosorbent preparation. The ability of the selective removal of Lp(a) from human plasma by this sorbent was demonstrated in an in vitro study. The material desorbated from the anti-Lp(a) column by acid pH has immunochemical properties identical to those of Lp(a) isolated by ordinary methods. The binding capacity of anti-Lp(a) sorbent is 3 mg Lp(a) per 1 ml gel. Immunosorbent prepared in this study was reusable. It is suggested that anti-Lp(a) immunosorbent may be applied for clinical plasma adsorption therapy for the Lp(a)-apheresis procedure, especially for patients with high Lp(a) concentration in blood and with stenosis in coronary and cerebral arteries.Keywords
This publication has 13 references indexed in Scilit:
- cDNA sequence of human apolipoprotein(a) is homologous to plasminogenNature, 1987
- Low-Density Lipoprotein Apheresis and Regression of Atherosclerotic Plaque In VitroArtificial Organs, 1986
- The association between serum Lp(a) concentrations and angiographically assessed coronary atherosclerosisDependence on serum LDL levelsAtherosclerosis, 1986
- Levels of lipoprotein Lp(a) decline with neomycin and niacin treatmentAtherosclerosis, 1985
- Reduction of lecithin-cholesterol acyltransferase, apolipoprotein D and the Lp(a) lipoprotein with the anabolic steroid stanozololBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- Heterogeneity of human plasma lipoprotein (a). Isolation and characterization of the lipoprotein subspecies and their apoproteins.Journal of Biological Chemistry, 1984
- Protein composition of Lp(a) lipoprotein from human plasmaFEBS Letters, 1983
- Lipoprotein Lp(a) and the risk for myocardial infarctionAtherosclerosis, 1981
- Immunochemical quantification of human plasma Lp(a) lipoproteinLipids, 1974