Methylglyoxal-induced DNA-protein cross-links and cytotoxicity in Chinese hamster ovary cells
- 1 January 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 6 (5) , 683-686
- https://doi.org/10.1093/carcin/6.5.683
Abstract
The technique of alkaline elution was applied to study the capacity of methylglyoxal to induce DNA damage and rapair in Chinese hamster ovary cells. DNA cross-linking was observed after a 90-min exposure to a subtoxic dose (1.5 mM), and the cross-links were fully repaired by 24 h. The cross-linking appeared to be DNA-protein in nature, since proteinase treatment removed the effect. When the same cells were exposed to methylgloxal in the presence of a rat liver metabolic system, both cytotoxicity and cross-linking frequency were significantly reduced.This publication has 15 references indexed in Scilit:
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