Autoregulation of I kappa B alpha activity.
- 4 January 1994
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 91 (1) , 28-32
- https://doi.org/10.1073/pnas.91.1.28
Abstract
Transcription factor NF-kappa B regulates the expression of a plethora of genes. The activity of NF-kappa B proteins is regulated by I kappa B proteins. We report that induction of I kappa B alpha, a member of the I kappa B family of proteins, is preceded by activation of NF-kappa B complex. The promoter of the I kappa B alpha gene contains a kappa B site that is directly involved in its induction by the NF-kappa B complex. Degradation of I kappa B alpha protein precedes activation of NF-kappa B DNA binding activity, whereas newly synthesized I kappa B alpha protein inhibits NF-kappa B activity. If the degradation of I kappa B alpha is prevented, the induction of DNA binding activity of NF-kappa B complex is severely curtailed. These data suggest the existence of an autoregulatory loop whereby I kappa B alpha regulates the activity of transcription factor NF-kappa B, which in turn regulates the I kappa B alpha activity.Keywords
This publication has 36 references indexed in Scilit:
- The inducible transcription activator NF-κB: regulation by distinct protein subunitsBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1991
- DNA binding and IκB inhibition of the cloned p65 subunit of NF-κB, a rel-related polypeptideCell, 1991
- NF-κB, KBF1, dorsal, and related mattersCell, 1990
- Cloning of the p50 DNA binding subunit of NF-κB: Homology to rel and dorsalCell, 1990
- The DNA binding subunit of NF-κB is identical to factor KBF1 and homologous to the rel oncogene productCell, 1990
- Activation in vitro of NF-κB" by phosphorylation of its inhibitor IκB"Nature, 1990
- NF-κB: A pleiotropic mediator of inducible and tissue-specific gene controlCell, 1989
- Activation of HIV gene expression during monocyte differentiation by induction of NF-kBNature, 1989
- The v-rel oncogene product is complexed to a 40-kDa phosphoprotein in transformed lymphoid cells.Proceedings of the National Academy of Sciences, 1988
- Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-κB transcription factorCell, 1988