Time-resolved fluorescence from biological systems: tryptophan and simple peptides
- 11 November 1980
- journal article
- Published by The Royal Society in Philosophical Transactions of the Royal Society of London. Series A, Mathematical and Physical Sciences
- Vol. 298 (1439) , 321-334
- https://doi.org/10.1098/rsta.1980.0257
Abstract
Four methods of applying mode-locked lasers to time-resolved fluorescence measurements in the subnanosecond region are compared. When time resolution below 100 ps is not required, the most precise and sensitive method is single-photon counting, and the application of this method to studies of time-resolved fluorescence of tryptophan in simple peptides is described. The dependence of lifetimes on pH and temperature are interpreted in terms of quenching by intramolecular proton and electron transfers.Keywords
This publication has 0 references indexed in Scilit: