KARYOKINESIS OF SOMATIC NUCLEI OFNEUROSPORA CRASSA: II. DNA REPLICATION IN SYNCHRONOUSLY DIVIDING CONIDIAL NUCLEI

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Abstract
DNA synthesis in synchronously dividing conidial nuclei of Neurospora crassa shows a drop after commencement of incubation. This drop persists for one hour. The magnitude of the loss of DNA over this period of time is correlated with the percentage of nonviable conidia in the sample suspension. After one hour of incubation DNA synthesis increases sharply but slackens after a further two hours. During this time interval, nuclei from hyphal fragments, and conidiophores commence karyokinesis. Conidia, on the other hand, are delayed in karyokinesis and hence DNA synthesis lags when compared with that of conidiophores and hyphal fragments. DNA synthesis in conidia is correlated with the appearance of late Filament and early Ring phase. Anaerobic conditions influence DNA synthesis as well as growth in terms of dry weight; however, the increase in DNA is sufficient to allow karyokinesis to take place.