Studies on the Hydrolysis of 1-Alkyl-sn-glycero-3-phosphoethanolamine in Subcellular Fractions of Rat Brain

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Abstract
Formation of 1-alkylglycerol from 1-alkyl-sn-glycero-3-phosphoethanolamine in different cell fractions of rat brain is reported. Substrates used were labeled either with 14C or 3H in the alkyl residue or with 14C in the alkyl and 3H in the ethanolamine residue. Examination of the lipid- and water-soluble cleavage products showed that both ethanolamine and phosphoethanolamine are liberated from the substrate in the microsomal fraction of 14-day-old rat brain. The latter product is rapidly hydrolyzed. In comparison with other cell fractions, the microsomes contained the highest enzyme activities, which exhibited a pH optimum of 7.1-7.5. SH-group reagents are inhibitors, whereas diisopropylfluorophosphate has no effect. As the animals age, these enzyme activities decrease in brain homogenates.