Conditioned media of Kupffer and endothelial liver cells influence protein phosphorylation in parenchymal liver cells. Involvement of prostaglandins
- 1 June 1988
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 252 (2) , 601-605
- https://doi.org/10.1042/bj2520601
Abstract
The possible role of Kupffer and endothelial liver cells in the regulation of parenchymal-liver-cell function was assessed by studying the influence of conditioned media of isolated Kupffer and endothelial cells on protein phosphorylation in isolated parenchymal cells. The phosphorylation state of three proteins was selectively influenced by the conditioned media. The phosphorylation state of an Mr-6300 protein was decreased and the phosphorylation state of an Mr-47000 and an Mr-97,000 protein was enhanced by these media. These effects could be mimicked by adding either prostaglandin E1, E2, or D2. Both conditioned media and prostaglandins stimulated the phosphorylase activity in parenchymal liver cells, suggesting that the Mr-97,000 phosphoprotein might be phosphorylase. Parenchymal liver cells secrete a phosphoprotein of Mr-63,000 and pI 5.0-5.5. The phosphorylation of this protein is inhibited by Kupffer-and endothelial-liver-cell media, and prostaglandins E1, E2 and D2 had a similar effect. The data indicate that Kupffer and endothelial liver cells secrete factors which influence the protein phosphorylation in parenchymal liver cells. This forms further evidence that products from non-parenchymal liver cells, in particular prostaglandin D2, might regulate glucose homoeostasis and/or other specific metabolic processes inside parenchymal cells. This stresses the concept of cellular communication inside the liver as a way by which the liver can rapidly respond to extrahepatic signals.This publication has 26 references indexed in Scilit:
- Mechanism of glucagon stimulation of fructose‐1,6‐bisphosphatase in rat hepatocytesFEBS Letters, 1986
- Stimulation of hepatic glycogenolysis by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) via cyclooxygenase productsBiochemical and Biophysical Research Communications, 1985
- Possible involvement of cyclooxygenase products in the actions of platelet-activating factor and of lipoxygenase products in the vascular effects of epinephrine in perfused rat liverBiochemical and Biophysical Research Communications, 1984
- Synthesis of prostanoids and cyclic nucleotides by phagocytosing rat Kupffer cellsEuropean Journal of Biochemistry, 1984
- Phorbol esters inhibit alpha1 adrenergic stimulation of glycogenolysis in isolated rat hepatocytesBiochemical and Biophysical Research Communications, 1984
- Synergistic activation of rat hepatocyte glycogen phosphorylase by A23187 and phorbol esterBiochemical and Biophysical Research Communications, 1984
- [4] Measurement of hormone-stimulated protein phosphorylation in intact cellsPublished by Elsevier ,1983
- Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study.The Journal of cell biology, 1977
- Chapter 4 Preparation of Isolated Rat Liver CellsPublished by Elsevier ,1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970