Catalytic Role of 2′-Hydroxyl Groups Within a Group II Intron Active Site
- 8 March 1996
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 271 (5254) , 1410-1413
- https://doi.org/10.1126/science.271.5254.1410
Abstract
Domain 5 is an essential active-site component of group II intron ribozymes. The role of backbone substituents in D5 function was explored through synthesis of a series of derivatives containing deoxynucleotides at each position along the D5 strand. Kinetic screens revealed that eight 2'-hydroxyl groups were likely to be critical for activity of D5. Through two separate methods, including competitive inhibition and direct kinetic analysis, effects on binding and chemistry were distinguished. Depending on their function, important 2'-hydroxyl groups lie on opposite faces of the molecule, defining distinct loci for molecular recognition and catalysis by D5.Keywords
This publication has 32 references indexed in Scilit:
- Two Competing Pathways for Self-splicing by Group II Introns: A Quantitative Analysis ofin VitroReaction Rates and ProductsJournal of Molecular Biology, 1996
- Model for an RNA tertiary interaction from the structure of an intermolecular complex between a GAAA tetraloop and an RNA helixNature, 1994
- Dissection of the Role of the Conserved G.cntdot.U Pair in Group I RNA Self-SplicingBiochemistry, 1994
- Building a Kinetic Framework for Group II Intron Ribozyme Activity: Quantitation of Interdomain Binding and Reaction RateBiochemistry, 1994
- Involvement of a GNRA tetraloop in long-range RNA tertiary interactionsJournal of Molecular Biology, 1994
- GAAA Tetraloop and Conserved Bulge Stabilize Tertiary Structure of a Group I Intron DomainJournal of Molecular Biology, 1994
- Tertiary interactions with the internal guide sequence mediate docking of the P1 helix into the catalytic core of the Tetrahymena ribozymeBiochemistry, 1993
- RNA substrate binding site in the catalytic core of the Tetrahymena ribozymeNature, 1992
- Functional contacts of a transfer RNA synthetase with 2′-hydroxyl groups in the RNA minor grooveNature, 1992
- Site-Specific Modification of Pre-mRNA: the 2′-Hydroxyl Groups at the Splice SitesScience, 1992