Using ELISA and PCR to test the potential for spread of plum pox virus by seeds of different stone fruit cultivars
- 1 March 2003
- journal article
- other
- Published by Taylor & Francis in New Zealand Journal of Crop and Horticultural Science
- Vol. 31 (1) , 69-72
- https://doi.org/10.1080/01140671.2003.9514237
Abstract
The enzyme‐linked immunosorbent assay (ELISA) and the polymerase chain reaction (PCR) were used to test the potential for spread of plum pox virus (PPV) by seeds of the apricot (Prunus armeniaca) varieties ‘Tyrinthos’ and ‘Bebecou’, and the stone fruit rootstocks ‘GF305’ (Prunus persica) and ‘Myrobalan’ (Prunus cerasifera). Seeds originating from infected ‘Tyrinthos’, ‘Bebecou’, ‘GF305’, and ‘Myrobalan’ trees were stratified in metal boxes containing sand in a screenhouse. Leaves were collected from each of the seedlings and tested for PPV. No seedling was found infected with this pathogen. Our results suggest that at least in the cultivars tested, PPV is not seed transmitted.Keywords
This publication has 10 references indexed in Scilit:
- A polymerase chain reaction assay adapted to plum pox potyvirus detectionPublished by Elsevier ,2002
- Alfalfa mosaic and pea seed-borne mosaic viruses in cool season crop, annual pasture, and forage legumes: susceptibility, sensitivity, and seed transmissionAustralian Journal of Agricultural Research, 2001
- Bean yellow mosaic potyvirus infection of alternative annual pasture, forage, and cool season crop legumes: susceptibility, sensitivity, and seed transmissionAustralian Journal of Agricultural Research, 2000
- Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISAPublished by Elsevier ,1998
- Multiple viral determinants affect seed transmission of pea seedborne mosaic virus in Pisum sativumJournal of General Virology, 1996
- DETECTION OF PLUM POX VIRUS USING A SIMPLIFIED POLYMERASE CHAIN REACTION PROCEDUREActa Horticulturae, 1995
- An eclipse of pea seed‐borne mosaic virus in vegetative tissue of pea following repeated transmission through the seedAnnals of Applied Biology, 1993
- Applied Plant VirologyPublished by Springer Nature ,1991
- The detection of plum pox virus in Prunus species by enzyme‐linked immunosorbent assay (ELISA)Annals of Applied Biology, 1978
- THE DETECTION OF PLUM POX AND OTHER VIRUSES IN WOODY PLANTS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)Acta Horticulturae, 1976