The Relationship of Rapidly Labeled Adrenal RNA Synthesis to Steroidogenesis in a Superfusion System: Effect of ACTH

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Abstract
A system of superfusion of rat adrenal glands with continuous collection of the incubation medium has been found to be useful for studies of the effect of ACTH on labelling of cytoplasmic RNA as well as upon corticosterone secretion. A continuous infusion of ACTH (24 mU/ml medium) maintained steroid output at a higher level than controls. The kinetics of incorporation of radioactive uridine into total cytoplasmic RNA indicated that at different time intervals the rate of synthesis of RNA was constant for 60 min of incubation. ACTH produced a rapid increase in the incorporation of radioactive precursor into total cytoplasmic RNA. A change in labeling patterns of cytoplasmic RNA species separated by polyacrylamide gel electrophoresis provided evidence for stimulation by ACTH of incorporation of radioactive precursor into rapidly labelled cytoplasmic RNA within 30 min. These results suggest that the continuous superfusion of adrenal glands provides a suitable method for studying the relationship between steroidogenesis and RNA synthesis. The stimulatory effect of ACTH on the synthesis of different fractions of cytoplasmic RNA is consistent with the known mechanism for ACTH-stimulated steroidogenesis and provides evidence for specificity of ACTH effects on cytoplasmic adrenal RNA. (Endocrinology93: 285, 1973)

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