Comparative studies on keratinase production of Trichophyton mentagrophytes strains of animal origin
- 1 May 1995
- Vol. 38 (5-6) , 205-209
- https://doi.org/10.1111/j.1439-0507.1995.tb00050.x
Abstract
Summary. Over a period of 28 days, 10 Trichophyton mentagrophytes strains were examined for their ability to secrete keratinolytic enzymes. Production of enzymes was stimulated by various keratins used as substrates. Duration and intensity of keratinase secretion were strongly influenced by the keratinous substrate. Duration and intensity of the enzyme production also differed among the 10 dermatophyte strains. Five different enzymes were isolated with molecular weights ranging from 28 kDa to 65 kDa. The different enzymes might be produced by different varieties of the species Trichophyton mentagrophytes.Zusammenfassung. Über einen Zeitraum von 28 Tagen wurden 10 Trichophyton mentagrophytes‐Stämme hinsichtlich ihrer Föhigkeit zur Sekretion keratinolytischer Enzyme untersucht. Die Keratinasebildung wurde durch verschiedene als Substrat verwendete Keratine stimuliert. Die Stärke und Dauer der Keratinasesekretion wurde sowohl von den verwendeten, Substraten als auch von den verschiedenen, untersuchten Stämmen beeinflußt. Es konnten 5 verschiedene Keratinasen mit Molekulargewichten zwischen 28 kDa und 65 kDa isoliert werden. Die Bildung verschiedener Keratinasen ist möglicherweise durch die Zugehörigkeit der Hautpilze zu verschiedenen Varietäten der Spezies Trichophyton mentagrophytes bedingt.Keywords
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