DEMONSTRATION OF CURDLAN-TYPE POLYSACCHARIDE AND SOME OTHER β-1, 3-GLUCAN IN MICROORGANISMS WITH ANILINE BLUE

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Abstract
Colonies of microorganisms capable of forming curdlan-type polysaccharide in glucose medium stained blue with aniline blue [AB], brilliant blue or trypan blue, and red with Congo red. Staining with AB was highly specific for colonies forming curdlan-type polysaccharide. AB was used to detect the distribution of curdlan-type polysaccharide and other .beta.-1,3-glucans in microorganisms. Of 687 strains of bacteria, 5 stains of Bacillus, Alcaligenes faecalis var. myxogens 10C3 and some strains derived from it, and some strains of Agrobacterium stained with AB. Sixteen of 60 strains of yeast also stained with this dye. No positive reaction was detected in 13 strains of fungi tested. This method was also useful for isolation of colonies forming only curdlan-type polysaccharide from organisms with the ability to form other water-soluble polysaccharides.

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