Mechanisms of Cell-to-Cell and Cell-to-Substrate Adhesion of Human Epithelial, Chang Conjunctiva Cells

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Abstract
The adhesive property was studied of Chang conjunctiva cells of human epithelial line. Cells dissociated with only EDTA aggregated in Ca2+-free and Mg2+-free conditions; trypsin-dissociated cells did not aggregate under the same conditions. Cells dissociated with trypsin solution containing Ca2+ aggregated in the presence of Ca2+ and/or Mg2+. Cells dissociated by trypsin without Ca2+ showed less aggregation in the presence of divalent cations. In cases of cell adhesion to noncellular substrates, the particular method of cell dissociation did not produce different results. Only a small fraction of inoculated cells attached onto plastic coated with bovine serum albumin or gelation. Many inoculated cells attached rapidly and spread onto plastic coated with collagen, when Mg2+ was present in the medium. Ca2+ and Mg2+ may act differently in cell adhesion.