Studies on an Inhibitor of Erythropoiesis I. Effects of Sera from Normal and Polycythemic Rabbits on Heme Synthesis in Rabbit Bone Marrow Cultures

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Abstract
Summary The effects of sera from normal and polycythemic rabbits on the rate of heme synthesis in erythroid cells were studied in vitro in rabbit bone marrow cultures. Sera from normal or hypertransfused rabbits significantly inhibited 59Fe incorporation into heme in normal rabbit bone marrow cultures when incubated alone or with erythropoietin (ESF) when compared with the response in controls with newborn calf serum alone or with ESF. A significantly greater inhibitory effect was seen with sera from polycythemic than from normal animals. This inhibitory activity increased exponentially with increasing concentrations of the test sera in the cultures, and was also directly proportional to the increase seen in the hematocrit of the polycythemic rabbits. These results suggest that the inhibitor in both normal and polycythemic sera is tissue-specific and may control the rate of heme synthesis and erythroid cell proliferation. This inhibitor is a low-molecular-weight substance.

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