Characterization of CMY-type beta-lactamases in clinical strains of Proteus mirabilis and Klebsiella pneumoniae isolated in four hospitals in the Paris area
Open Access
- 22 October 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 50 (5) , 681-688
- https://doi.org/10.1093/jac/dkf193
Abstract
We isolated five clinical strains (three Proteus mirabilis and two Klebsiella pneumoniae) with β-lactam resistance phenotypes consistent with production of an AmpC-type β-lactamase. The predicted amino acid sequences of the enzymes were typical of class C β-lactamases. The enzymes were identified as CMY-2, CMY-4 and a new CMY-variant β-lactamase, CMY-12. The AmpC β-lactamases from the two K. pneumoniae isolates were found to be encoded on self-transferable plasmids. The genes encoding the AmpC-type β-lactamase produced by the three P. mirabilis isolates were chromosomal. Four of the five clinical isolates were from patients transferred from Greece, Algeria and Egypt; one of the K. pneumoniae strains was recovered from a French patient. PFGE analysis and rep-PCR fingerprinting showed that the two P. mirabilis isolates from Greek patients were closely related.Keywords
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