Lipid glyceride synthesis by rat skeletal muscle

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Abstract
In rat diaphragm, glyceride synthesis was observed to occur predominantly in the microsome fraction. In a phosphate buffered system containing CoA, ATP, Mg++, α-glycerophosphate, fluoride, and mercaptoethanol, palmitate-1-C14 was incorporated into phosphatidic acid, diglyceride, and triglyceride. Some of the enzymatic characteristics of the isolated organelle system are reported. Skeletal muscle from the back and legs of rats possesses a similar capacity in the microsome fraction; the latter system incorporated oleate-1-C14, linoleate-1-C14 and linolenate-1-C14 into glycerides. Both types of skeletal muscle have also a soluble enzyme that can synthesize glycerides.