Simple modification of a protein database for mass spectral identification of N‐linked glycopeptides
- 29 September 2005
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 19 (21) , 3002-3006
- https://doi.org/10.1002/rcm.2162
Abstract
We describe an algorithm which modifies a protein database such that during a database search deamidation is limited to asparagines strictly contained within the N-glycosylation consensus sequence. The modified database was evaluated using a dataset created from the shotgun proteomic analysis of N-linked glycopeptides from human blood serum. We demonstrate that the application of the modified database eliminates incorrect glycopeptide assignments, reduces the peptide false-discovery rate, and eliminates the need for manual validation of glycopeptide identifications. Copyright © 2005 John Wiley & Sons, Ltd.Keywords
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