Use of a Modified Radioligand Assay to Measure the Effect of Estradiol on Uterine Adenosine 3',5'–Cyclic Monophosphate
- 1 February 1973
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 92 (2) , 494-499
- https://doi.org/10.1210/endo-92-2-494
Abstract
The acute effects of estrogen on castrate rat uterine cAMP were studied employing a well—validated assay procedure for the nucleotide. Estradiol (1 μAg/100 g bw) administered iv produced no alteration in cAMP from 0–30 min of exposure. Using a suspended uterine preparation in vitro, estradiol (0.5–10 μM) was again ineffective under conditions where isoproterenol produced a marked increase in cAMP concentration. It is concluded that acute elevation of cAMP is not a regular feature of estrogen action on the uterus. (Endocrinology92: 494, 1973)Keywords
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