Distribution of prostaglandin E2 and prostaglandin F2α receptors in human myometrium

Publisher Website
Google Scholar
Abstract
Human myometrium was studied for specific binding of PG[prostaglandin]E2 and PGF2.alpha.. PGF2.alpha.-binding was almost undetectable, but specific binding sites for PGE2 with high affinity (Kd = 2.7 .+-. 0.4 .times. 10-9 M) were demonstrated. The binding capacity for PGE2 exhibited a topically different distribution pattern with the highest values in the central parts and low to undetectable levels in the cervical region. Binding characteristics were analyzed by receptor kinetics, revealing a homogenous receptor population. Binding capacity in uteri obtained from post-menopausal women was of the order of 900-940 femtomole/mg protein. Estrogen pre-treatment and pregnancy were associated with a 3-fold reduction of the PGE2-binding capacity.