Degradation of Outer Membrane Protein A in Escherichia coli Killing by Neutrophil Elastase
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- 18 August 2000
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 289 (5482) , 1185-1187
- https://doi.org/10.1126/science.289.5482.1185
Abstract
In determining the mechanism of neutrophil elastase (NE)–mediated killing of Escherichia coli , we found that NE degraded outer membrane protein A (OmpA), localized on the surface of Gram-negative bacteria. NE killed wild-type, but not OmpA-deficient, E. coli . Also, whereas NE-deficient mice had impaired survival in response to E. coli sepsis, as compared to wild-type mice, the presence or absence of NE had no influence on survival in response to sepsis that had been induced with OmpA-deficient E. coli . These findings define a mechanism of nonoxidative bacterial killing by NE and point to OmpA as a bacterial target in host defense.Keywords
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