Antibody inhibition of ferripyochelin binding to Pseudomonas aeruginosa cell envelopes
- 1 October 1984
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 23 (21) , 5076-5080
- https://doi.org/10.1021/bi00316a038
Abstract
A 14,000 MW (14K) protein which binds ferripyochelin was purified from cell envelopes of P. aeruginosa low Fe grown cells. The purified protein migrated as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and was shown to be free of contamination by lipopolysaccharide or carbohydrate. Rabbit antiserum to this protein reacted with the purified protein by immunoblot assay. The IgG fraction of this antiserum blocked binding of [59Fe]pyochelin to isolated cell envelopes of P. aeruginosa in a dose-dependent fashion.This publication has 15 references indexed in Scilit:
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