The flow of blood cell suspensions through 3 μm and 5μm Nuclepore membranes: a comparison of kinetic analysis with scanning electron microscopic examinations

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Abstract
The effect of [human] red and white cells on the flow of dilute suspensions of blood cells through 3 .mu.m and 5 .mu.m Nuclepore membrane filters was investigated. The rate of flow of blood cell suspensions through 3 .mu.m and 5 .mu.m membranes declines continually due to occlusion of pores by slow cells. The cells which occlude 3 .mu.m pores exceed the number of white cells about 7-fold. EM examination of a used membrane confirms that these slow cells are not white cells but are red cells which are visibly damaged. With 5 .mu.m membranes, the number of slow cells is entirely consistent with them being white cells. Again, EM examination confirms that 5 .mu.m pores are occluded by white cells. With both types of membrane, the same kinetic analysis is valid and yields information about the behavior of red cells during the filtration procedure.